A Comparative Study of Laboratory and Commercially Prepared Pregnancy Tests.
نویسندگان
چکیده
LABORATORY METHOD Antiserum to human chorionic gonadotrophin was prepared by giving rabbits an intravenous injection of 15,000 i.u. human chorionic gonadotrophin (Pregnyl, Organon) in 2 ml. of a 1% bentonite suspension in normal saline (Butt, Crooke, and Cunningham, 1961) at weekly intervals for five weeks. The rabbits were bled one week after the final injection of human chorionic gonadotrophin. Sheep red cells were tanned by the modification of Ling (1960), as described by Butt et al. (1961). Pyruvic aldehyde reagent was prepared by adding 16 vol. 25% pyruvic aldehyde solution to 3-0 vol. saline, and the pH adjusted to 7 0 with 10% sodium carbonate solution. To this reagent was added 0-7 vol. 0-15 M phosphate buffer (pH 8 0), and 1-0 vol. of a 50% suspension of the washed red cells in saline. The mixture was stored at 4°C. for two days with occasional shaking. The cells were then washed at least six times with saline and stored as a 10% suspension containing 0-1 % sodium azide as a preservative. Inefficient washing ofthe stabilized cells will result in the formation of an ill-defined pattern in the agglutination reaction. To sensitize the cells, 2 ml. of the 10% suspension was centrifuged, the supernatant discarded, and the cells washed twice in saline. Buffered saline, 10 ml. (saline, 10 vol.; 0 15 M Na2HPO4, 3 5 vol.; 0-15 M KH2P04, 6-5 vol.), containing 1,500 i.u. human chorionic gonadotrophin was added to the cells and the mixture incubated at 37°C. for one hour. The cells were then washed twice in saline and stored at
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ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 16 شماره
صفحات -
تاریخ انتشار 1963